Under this circumstance, the equally pooled prevalence of HEV antigen and RNA in our study can suggest that the anti-HEV IgG and IgM testing may not help to eliminate HEV infectious blood donation because IgG is a marker of previous infection and IgM disappears early in the convalescent period which may further propose HEV RNA detection as “golden standard” for detecting HEV infectious blood donors[43]. This evidence concerns the gene CD40LG and infection.