The estimates for the two loss-of-function PALB2 variants (c.1592delT and c.3113G<A) were consistent with each other and with estimates based on segregation analysis.5, 6, 9 We found no evidence of association with breast cancer for PALB2 c.2816T>G (p.Leu939Trp), with an upper 95% confidence limit excluding an OR >1.5 which is notable given the Align-Grantham Variation Granthan Deviation (Align-GVGD) score and the observed impact on protein function.30 The gene discussed is PALB2; the disease is breast cancer.