To explore the methods to improve the sensitivity of ESCC cells to rapamycin, in the present study, the expression of p70S6K and p-p70S6K in EC9706 cells was downregulated by p70S6K siRNA; the changes of cell sensitivity to rapamycin in cell proliferation, cell cycle, and tumor growth were investigated in vitro and in vivo. The gene discussed is RPS6KB1; the disease is esophageal squamous cell carcinoma.