After cells were treated with rapamycin, the expression of p-p70S6K was obviously reduced in the three ESCC cell lines, especially in EC9706 cells, and rapamycin obviously inhibited the expression of p-p70S6K at a short time (1 hour) compared to that in the other two ESCC cell lines (P < 0.001, Figure 1). Here, RPS6KB1 is linked to esophageal squamous cell carcinoma.