CDKN2B-AS1 and coronary artery disorder: This difference in the direction of associations observed between the methylation of the individual CpG sites within the ANRIL promoter and CHD risk factors may reflect the fact that gene promoter DNA methylation often influences expression through the modulation of transcription factor binding [24], and while some studies have shown that the methylation status of CpG sites can be closely aligned to that of their neighbours, especially within a CpG island, developmentally induced changes are often CpG site specific [25].