TARDBP and amyotrophic lateral sclerosis: We next investigated if we were able replicate this in cell culture by extracting the sarkosyl insoluble fraction from cells transfected with the FL WT TDP-43 plus ALS tissue extracted pTDP-43 (ALS TCX), and then re-transfecting of 5 μg of the cell extract (SK) into naive cells expressing FL WT TDP-43 (Fig. 3).