Whilst ALS brain tissue has been previously seeded (Nonaka et al., 2013), we have developed a TDP-43 aggregation model that can be reproduced directly from pTDP-43 extracts from both the brain and spinal cord of patients with ALS, the latter, arguably being the more relevant pathological tissue in ALS, and which has not been previously demonstrated. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.