To investigate whether FGF2 produced by fibroblasts upon A2BR stimulation could influence tumor cell proliferation, we performed MTT assays in B16.F10 melanoma cells co-cultured with tumor-isolated fibroblasts stimulated with 10 nM Bay60-6583 or vehicle (Ctr) for 24 hours or 48 hours. This evidence concerns the gene FGF2 and melanoma.