The tinnitus group had significantly higher mRNA expression of KCC2 gene than the control group in the frontal lobes (0.85 ± 0.23 versus 0.46 ± 0.12, p = 0.003), and the spirulina group had significantly lower mRNA expression of KCC2 gene than the tinnitus group in the temporal lobes (0.45 ± 0.11 versus 0.85 ± 0.23, p = 0.002). The gene discussed is SLC12A5; the disease is Tinnitus.