ZEB1 and central nervous system cancer: Furthermore, we measured the expression levels of ZEB1-AS1 in glioblastoma cell lines and normal brain tissues with qRT-PCR, finding that they were significantly higher in three high-degree glioblastoma cell lines (T98G, U87, U251) than in the low-degree glioma cell line (HS683) and normal brain tissues (Figure 1B).