Flow cytometry analysis revealed that knocking down ZEB1-AS1 in U87 and U251 cells significantly enhanced the cell apoptosis rate in comparison with NC groups (Figure 3B, p < 0.05 for U87, p < 0.05 for U251), indicating the crucial role of ZEB1-AS1 in the apoptosis regulation of human glioma cells. This evidence concerns the gene ZEB1 and glioma.