Aside from these findings, several potential limitations of this present study exist, due to other possible underlying mechanisms in the ischemic stroke-induced BBB dysfunction, including the phosphatidylinositide 3-kinases/protein kinase B (PI3K/Akt), NF-κB and rho-associated coiled coil-containing protein kinase-1/myosin light chain (ROCK1/MLC) signaling pathways [43,44,45]. This evidence concerns the gene ROCK1 and ischemic stroke.