Moreover, ChIP assays revealed that the expression of Nrf2 significantly affected Sp1 binding to PDGFA promoter in Nrf2 over-expressed or Nrf2-silenced MHCC-97H cells (Figure 4K), indicating that, apart from Sp1, the constitutive level of Nrf2 in HCC cells was mandatory to manipulate PDGFA transcription. This evidence concerns the gene PDGFA and hepatocellular carcinoma.