According to eIF4E expression in esophageal cell lines, qPCR showed that eIF4E was highly expressed in all three cancer cell lines (P < 0.05 for EC-1 & EC109 vs. HEEpic, and P < 0.01 for EC9706 vs. HEEpic) and EC9706 has the highest eIF4E expression (P < 0.05 for EC9706 vs. EC-1 or EC109) among these 3 ESCC cell lines, whereas it was weakly detected in Human Esophageal Epithelial Cells HEEpic (Figure 2A). This evidence concerns the gene EIF4E and esophageal squamous cell carcinoma.