We first profiled several GBM cell lines (U87 and GS2), GBM stem cells grown (GSC 8–18 and GSC 7–2) and two newly established GBM primary cultures growth in glioma stem cell conditions (TWH1 and TWH2) for several glycolysis markers, HK2, PDK1, LDHA, and GLS, the enzyme responsible for conversion of glutamine to glutamate (Figure 8A). The gene discussed is GLS; the disease is glioma.