HIF1A and neoplasm: In this study, we show that through a two-stage-induction method which included the knockdown of hypoxia inducible factor 1a (HIF1a) gene expression and then sequential induction by 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3, VD3) combined with suberoylanilide hydroxamic acid treatment (VD3/SAHA) in the second stage, the LACSCs changed to differentiated state with disappearance of stem cell markers; diminished ability to invade and clonogenic capacity in vitro; regained sensitivity to cisplatin; lost tumorigenic capacity and decreased tumor cell proliferation in vivo.