LGALS4 and breast cancer: We monitored spontaneous neural activities among BC populations by performing in vivo time-lapse two-photon calcium imaging of double transgenic Tg(Gal4-VP16xfz43,UAS:GCaMPHS) or Tg(Gal4-VP16xfz43,UAS:GCaMP1.6) zebrafish larvae, in which BCs express the genetically encoded calcium indicator GCaMPHS or GCaMP1.6 (Supplementary Fig. 1a,b, and see also ref. 26, 27).