Given the finding that neither antibiotics nor miR-204 inhibition impacted endothelium-independent vasorelaxation (Supplementary Fig. 12), it is likely that targets of miR-204, other than Sirt1, that are partly responsible for miR-204-mediated endothelial dysfunction with HFD feeding are also expressed in the endothelium. This evidence concerns the gene SIRT1 and endothelial dysfunction.