Using a NF-κB luciferase reporter vector (Fig. 3A), we found that LPS treatment induced a significant activation of NF-κB in human colon cancer cell line HT29 cells, and overexpression of CK8 significantly attenuated the NF-κB activation that was induced by LPS in a dose-dependent manner (Fig. 3B). The gene discussed is KRT8; the disease is malignant colon neoplasm.