The markers of HCC stem cells, CD90 and CD133, were used to demonstrate GRAMD1A regulates HCC stem cells, western blot assay found overexpression of GRAMD1A increased CD90 and CD133 levels, while knockdown of GRAMD1A decreased CD90 and CD133 levels (Fig. 2d). This evidence concerns the gene PROM1 and hepatocellular carcinoma.