This result may be explained by the findings of Tenneti et al. showing S-nitrosylation mediated by NO can inhibit the activation process of procaspases or inactivate caspases itself.35 Flow cytometry as well as TUNEL assay could not show rising cell numbers undergoing apoptosis exhibiting annexin V on the surface and fragmented DNA in nuclei in U87, LN229 and primary IC glioma cells. The gene discussed is ANXA5; the disease is central nervous system cancer.