CCND2 and renal cell adenocarcinoma: If the down-regulation/silence of CCND2 was regulated by promoter methylation, then demethylation of the gene by exposure to DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza) or treatment with histone deacetylase inhibitor trichostatin A (TSA) should result in the up-regulation of CCND2. Indeed, when six RCC cell lines were treated with 5-Aza with or without TSA, five out of six cell lines showed a differently increased expression of CCND2 (as analyzed by RT-PCR and Real-time PCR, Fig 2A and 2C) and partially demethylated (as analyze by MSP, Fig 2B).