MTOR and glioblastoma: At the concentration examined, TMZ alone and in combination with TMZ caused a minimal increase in apoptosis, but had no clear G2/M-blocking effect; this contrasts with a previous report showing that an AMPK agonist blocks G2/M in cells (Figure 3E) [16] An examination of GBM-TSs revealed inconsistent expression of AMPK-mTOR pathway proteins.