To further characterize the mechanisms of the antiproliferative activity of DFX, the pancreatic cancer cell lines BxPC-3, HPAF-II, and Panc 10.0 were incubated with either the vehicle control (PBS) or concentrations of 10, 50, or 100 μM of DFX for 72 h, and apoptosis was examined by flow cytometry using PI and Annexin V staining. This evidence concerns the gene ANXA5 and pancreatic neoplasm.