To identify potential autologous glycan-free V5 loops, a panel of ∼150 CAP257 Env sequences isolated by single-genome amplification (SGA) at 16 time points throughout infection of donor CAP257 were examined, but all the sequences were glycosylated, making it unclear which virus stimulated the CAP257-RH lineage (see Fig. S4 in the supplemental material). This evidence concerns the gene ERVW-1 and infection.