NR3C1 and osteosarcoma: Therefore, we first grouped genes regulated by GR in U2OS cells15, a human osteosarcoma cell line, into strong responders (top 20% with greatest fold induction on dexamethasone treatment, 290 genes) and a control group of weak responders (genes with significant changes in expression, log2-fold change <0.72, 688 genes) (Fig. 2a).