PLCγ1 knockdown decreased IP3 production and intracellular Ca2+ [Ca2+]i peak amplitude in normal T-cells upon anti-CD3 stimulation (Supplementary Fig. 5a), but no significant change was observed in primary T-ALL cells (Supplementary Fig. 5b) or Jurkat T-cells (Supplementary Fig. 5c). Here, PLCG1 is linked to acute lymphoblastic leukemia.