By AFAP1-AS1-specific siRNA (si-AFAP1-AS1) transfection, AFAP1-AS1 expression can reduce 56 % of mock treated SW480, and this AFAP-AS1 silencing resulted in suppression of CRC cell proliferation, indicating by MTT assay and colony formation assay. The gene discussed is AFAP1; the disease is colorectal carcinoma.