In this study, they found that AFAP1-AS1 knockdown could induce the loss of stress filament integrity and affect the expression of Rho/Rac GTPase family members and actin cytokeratin signaling pathway proteins in nasopharyngeal cancer cells, so we speculate that AFAP1-AS1 knockdown could also affect actin cytoskeleton via small Rho GTPase in colorectal cancer cell, we will confirm it in the further study. The gene discussed is RHO; the disease is colorectal cancer.