In this study, they found that AFAP1-AS1 knockdown could induce the loss of stress filament integrity and affect the expression of Rho/Rac GTPase family members and actin cytokeratin signaling pathway proteins in nasopharyngeal cancer cells, so we speculate that AFAP1-AS1 knockdown could also affect actin cytoskeleton via small Rho GTPase in colorectal cancer cell, we will confirm it in the further study. This evidence concerns the gene PTGDR and colorectal cancer.