It is worth to mention that both capture systems showed a scarce coverage in TP53, one of the most frequently mutated genes in cancer [37, 38], with only 3/8 amplicons with a read depth greater than 20× (Agilent: Chr17:7576996-7577178; Chr17:7578160-7578320; Chr17:7578335-7578503; Roche NimbleGen: Chr17:7577489-7577636; Chr17:7578160-7578320; Chr17:7579330-7579506) (Fig. 6, Additional file 3: Table S7). The gene discussed is TP53; the disease is cancer.