Two SNVs (NM_000455.4 (STK11): c.157G > C, p.Asp53His; NM_000546.5 (TP53): c.476C > A, p.Ala159Asp) were only identified in two FFPE samples (Additional file 3: Table S8) suggesting an intra-tumor heterogeneity as commonly described in lung cancer [39]. This evidence concerns the gene TP53 and lung cancer.