In the present study, we explored 1) the differential protein profile related to rats with silicosis that were treated with Ac-SDKP; 2) the expression dynamics of α-Ac-Tub in the rat silicosis model; and 3) the differential expression of α-Ac-Tub regulated by HDAC6 and α-TAT1 in terms of the anti-silicosis effect of Ac-SDKP, particularly the inhibition of myofibroblast differentiation induced by Ang II. This evidence concerns the gene HDAC6 and silicosis.