The expression of AFAP1‐AS1 in 65 paired ESCC tissues and corresponding adjacent tissues was observed by qRT‐PCR, which showed that AFAP1‐AS1 expression in ESCC was significantly elevated in 73.84%(48 of 65, fold ≧1.0) (P < 0.01) (Fig. 1A and B). Here, AFAP1 is linked to esophageal squamous cell carcinoma.