After cloning NCS1 Var1 and NCS1 Var2 into the mammalian expression vector pcDNA 2.1/3.1, Western blots of transiently transfected SHSY5Y, a human neuroblastoma cell line, showed expression of two distinct bands representing the two isoforms (Fig 1D, left panel). Here, NCS1 is linked to neuroblastoma.