In 1.7% of the samples, the DTU (n = 109) was reported as a group of DTUs: (i) in one dog, 15 coati from Brazil, and ten triatomines from Argentina, TcII, TcV, and TcVI were not distinguished; (ii) TcII or TcVI was reported in two T. infestans from Paraguay; (iii) 47 infections with TcV or TcVI in dogs, humans, T. infestans from Chile and Bolivia and P. megistus in Brazil were reported; (iv) in 31 vectors and mammal hosts from Brazil and Mexico TcIII/TcIV were not discriminated; and (v) in three cases TcI and TcII were not discriminated in T. pallidipennis. This evidence concerns the gene TCN2 and infection.