In osteosarcoma cells, curcumin, the active ingredient of turmeric, demonstrated a significant suppression of extrinsic activation of β-catenin/TCF transcriptional activities using either GSK-3β inhibitor (BIO), wild type β-catenin plasmid or mutant S33Y β-catenin plasmid (containing gene of mutant β-catenin which is resistant to Axin-APC-GSK3β destructive complex) in a dose dependent manner. Here, GSK3B is linked to osteosarcoma.