Efficient knockdown of C5orf45 was additionally confirmed in HeLa cervical carcinoma cells using two individual siRNAs that also resulted in an increased proportion of cells with γH2AX and 53BP1 foci (Figures 1A and 1B, respectively), thus validating the initial screen results and reducing the possibility of an off-target effect from a single siRNA. This evidence concerns the gene MRNIP and cervical carcinoma.