LGR5 and adenoma: In order to test whether recombination could be further induced, we re-challenged animals with ApcΔ/ΔCdh1fl/flLgr5CreERT2 adenoma with a second series of tamoxifen injections, in the expectation that as Lgr5 expression is also activated by the Wnt pathway, repeated recombination would increase the detection of Cdh1Δ/Δ within adenoma, [14, 34].