In the case of HBV-associated HCC, specific siRNAs were designed against splicing factors U2AF2, SF3A2, RBM8A, RBM4, PRPF4B, NOVA1, KHSRP, HNRPU, HNRPL, HNRPH1, HNRPC, and HNRPA1. The gene discussed is NOVA1; the disease is hepatocellular carcinoma.