In order to determine if AT1R or AT2R is involved in angiotensin-II-induced ESCC cell proliferation, CE81T/VGH cells were treated with an AT1R antagonist (irbesartan or losartan) or an AT2R antagonist (PD123319) for 30 min prior to the angiotensin II treatment. Here, AGTR1 is linked to esophageal squamous cell carcinoma.