In order to determine if AT1R or AT2R is involved in angiotensin-II-induced ESCC cell proliferation, CE81T/VGH cells were treated with an AT1R antagonist (irbesartan or losartan) or an AT2R antagonist (PD123319) for 30 min prior to the angiotensin II treatment. The gene discussed is AGT; the disease is esophageal squamous cell carcinoma.