CE81T/VGH and CE48T/VGH cells were treated with everolimus (an mTOR inhibitor) prior to the angiotensin II treatment, and everolimus significantly decreased the level of angiotensin II-induced ESCC cell proliferation, colony formation, and BrdU incorporation (Figure 4B). The gene discussed is MTOR; the disease is esophageal squamous cell carcinoma.