Figure 5A shows representative sections of the expression of p-Jak2 and p-Stat3. The IHC images showed that expression of p-Jak2 and p-Stat3 were elevated in the colon tissues of mice treated with AOM/DSS, which was markedly decreased after mice were treated with LFs. Moreover, Western blot analyses were consistent with IHC evaluation (Figure 5B,C), verifying that high expression of p-Jak2 and p-Stat3 induced by AOM/DSS was substantially suppressed by LFs treatment. This evidence concerns the gene STAT3 and infectious otitis media.