Six classical CIMP markers proposed by Issa et al. or Laird et al., i.e. CACNA1G (also known as MINT31), p16INKA, NEUROG1, MINT17, MLH1, and TIMP3 [11, 13, 29, 30], were analyzed in this study, and all of these markers were mostly unmethylated in FAP tumors, indicating CIMP(-). This evidence concerns the gene TIMP3 and Familial adenomatous polyposis.