The antitumor activity of MTI-31 in MDA-MB-453 tumors correlated well with its inhibition of mTORC1 and mTORC2 biomarkers in the tumor tissues as shown by a significantly reduced P-S6 and P-AKT levels at 5 mg/kg and a nearly complete suppression of both biomarkers at 10 mg/kg (Figure 2C, 2D). This evidence concerns the gene AKT1 and neoplasm.