To investigate whether HF can protect against HIV-1 Tat-induced changes in LRP1 and RAGE expression, hCMEC/D3 cells were cotreated with HF and HIV-1 Tat for 24 h (for western blotting and immunofluorescence staining) or for 12 h (for real-time RT-PCR). The gene discussed is LRP1; the disease is hydrops fetalis.