Although the negative surface charge of Dsi RNPs, as shown in the Fig. 2E, does not allow Dsi RNPs to readily cross the anionic cell membrane through passive diffusion, the folate receptor-mediated cellular binding of Dsi RNPs are expected to increase their uptake on the cancer cells especially with the overexpressed folate receptors, as opposed to the folate-free Dsi RNPs. This evidence concerns the gene P4HB and cancer.