PFKP and melanoma: This finding, along with the result that expression of other enzymes upstream of PFKP in the glycolysis pathway remained unaffected by ABCB5 blockade, suggests that ABCB5 regulates the glycolysis pathway at the level of PFKP, thereby explaining the observed reduced levels of the glycolytic by-products pyruvate and lactate in ABCB5-KD melanoma cells.