ABCB5 and melanoma: In order to address the molecular mechanism underlying ABCB5-mediated regulation of these metabolic pathways, we blocked ABCB5 protein function in human G3361 melanoma cells through treatment with different concentrations of the anti-ABCB5 blocking monoclonal antibody (mAb) clone 3C2-1D12 (25 or 50 μg/ml), or, as controls, exposed cells to isotype control mAb or no treatment.