However, the most optimal diagnostic biosignature irrespective of HIV status was a combination between six markers (NCAM, SAP, IL-1β, sCD40L, IL-13 and Apo A-1), which diagnosed TB disease with a sensitivity 100% (95% CI, 86.3-100%) and specificity of 89.3% (95%CI, 67.6-97.3%) after leave-one-out cross validation. This evidence concerns the gene CD40LG and tuberculosis.