qRT-PCR analysis of the transcriptional profile of LmDEFs in both fat body and salivary glands under nosemosis suggested that Nosema development, supported by localizing the Nosema β-actin at day 10 post-infection, may have a growing impact on the immune gene expression changes evidenced by augmented transcript levels at day 15 compared to day 5. This evidence concerns the gene ACTB and infection.