Moreover, immunohistochemical analysis of 234 paired paraffin-embedded CRC specimens revealed that immunostaining of CHKA protein was observed mainly in the cytoplasm and 70.9% (166/234) of the cancerous tissues were classified as CHKA-positive, whereas only 15.8% (37/234) of the adjacent normal mucosa tissues showed positive CHKA immunoreactivity. This evidence concerns the gene CHKA and colorectal carcinoma.