The in vivo behavior of cells from a bone metastasis (BM) primary culture was compared with that of two different breast cancer cell lines: MCF7, a non-invasive, hormone receptor–positive line (like the BM primary culture), and MDA-MB 231, a highly invasive, triple-negative breast cancer line widely used for in vivo studies on breast cancer metastasis. Here, NR4A1 is linked to breast carcinoma.