The number of ESCC cells that invaded Transwell chambers pre-coated with matrigel was significantly reduces and the expression of cellular adhesion molecule E-cadherin which mediates homophilic calcium dependent intracellular adhesion was enhanced after a reduction of Ets2 in vitro and in vivo, illustrating Ets2 knockdown inhibited the invasion of ESCC cells via strengthen the cellular adhesion. Here, ETS2 is linked to esophageal squamous cell carcinoma.