In order to confirm whether the growth-inhibiting effect of Ets2 depletion is relevant to ESCC growth in vivo, silent expression of Ets2 were inoculated into BALB/C athymic mice after siRNA1 sequence was ligated into retroviral vectors to establish the Eca109 cell stably silencing Ets2. This evidence concerns the gene ETS2 and esophageal squamous cell carcinoma.