ETS2 and esophageal squamous cell carcinoma: To better understand the role of Ets2 in ESCC, three candidate siRNA fragments again Ets2 (marked as siRNA1, siRNA2, siRNA3) were synthesized to interfere Ets2 expression and the recombinant siRNA particles were transfected into ESCC cells with Lipofectamine® 2000 Reagent (Invitrogen) following the manufacturer's protocol.