Using Western blot analysis, we confirmed successful fractionation based on the distribution of lamin B and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) but were unable to detect an increase in either β-actin or LifeAct-GFP-NLS levels in the nucleus during infection, suggesting that HCMV likely induces nuclear F-actin from a preexisting pool of monomeric nuclear G-actin (see Fig. S3 in the supplemental material). This evidence concerns the gene GAPDH and infection.