Multivariate analysis after adjustment revealed that T stage, M stage, pretreatment CEA level, adjuvant chemotherapy and TILs-PD-1 were independent prognostic factors for OS, while only T stage, M stage and TILs-PD-1 were independent prognostic factor for DFS of CRC patients (Table 3). The gene discussed is PDCD1; the disease is colorectal carcinoma.