ANXA5 and breast cancer: We previously showed that BTCI significantly induces cell cycle arrest at G2/M phase, DNA fragmentation and enhanced numbers of cells marked by annexin-V in breast cancer MCF-7, suggesting apoptosis as a possible mechanism for the cell death process.13 To explore whether apoptosis is involved in BTCI-induced MDA.MB.231, the amount of sub-G1 DNA in cancer cells treated with BTCI was investigated.