TP53, Ras and PIK3CA have important role in glucose metabolism,12,42,43 and are commonly mutated in cancers.44–47 We summarized the mutations in these cell lines by referring to Catalogue of Somatic Mutation in Cancer (COSMIC), and noticed that c-MYC protein decreased in four cell lines with wild-type P53 (HEK293T, HeLa, MCF7, HCT116), and increased in majority cell lines with mutated P53 or P53-null after GD treatment (Table 1). This evidence concerns the gene PIK3CA and cancer.